Chronic exposure to deltamethrin disrupts intestinal health and intestinal microbiota in juvenile crucian carp.

Hao Wu, Jinwei Gao, Min Xie, Jiayu Wu, Rui Song, Xiping Yuan, Yuanan Wu, Dongsheng Ou

Ecotoxicology and environmental safety 2022 Aug

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The indiscriminate use of deltamethrin in agriculture and aquaculture can lead to residues increased in many regions, which poses negative impacts on intestinal health of aquatic organisms. Although the potential toxicity of deltamethrin have recently attracted attention, the comprehensive studies on intestinal injuries after chronic deltamethrin exposure remain poorly understood. Herein, in a 28-day chronic toxicity test, crucian carp expose to different concentrations of deltamethrin (0, 0.3, and 0.6 μg/L) were used as the research object. We found that the morphology changes and increased goblet cells in intestinal tissue, and the extent of tissue injury increased along with the increasing exposure dose of deltamethrin. Additionally, the genes expression of antioxidant activity (Cu/Zn superoxide dismutase (Cu-Zn SOD), glutathione peroxidase 1 (GPX1), and catalase (CAT)), inflammatory response (tumor necrosis factor alpha (TNFα), interferon gamma (IFNγ), and interleukin 1 beta (IL-1β)), and tight junctions (Claudin 12 (CLDN12), and tight junction protein 1 (ZO-1)) dramatically increased. Meanwhile, the apoptosis and autophagy process were triggered through caspase-9 cascade and autophagy related 5 (ATG5)- autophagy related 12 (ATG12) conjugate. Besides, chronic deltamethrin exposure increased the amount of Proteobacteria and Verrucomicrobiota, while decreased Fusobacteriota abundance, resulting in intestinal microbiota function disorders. In summary, our results highlight that chronic exposure to deltamethrin cause serious intestinal toxicity and results in physiological changes and intestinal flora disturbances. Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.

Citation

Hao Wu, Jinwei Gao, Min Xie, Jiayu Wu, Rui Song, Xiping Yuan, Yuanan Wu, Dongsheng Ou. Chronic exposure to deltamethrin disrupts intestinal health and intestinal microbiota in juvenile crucian carp. Ecotoxicology and environmental safety. 2022 Aug;241:113732