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    Carbon dioxide (CO2) has been increasingly regarded not only as a greenhouse gas but also as a valuable feedstock for carbon-based chemicals. In particular, biological approaches have drawn attention as models for the production of value-added products, as CO2 conversion serves many natural processes. Enzymatic CO2 reduction in vitro is a very promising route to produce fossil free and bio-based fuel alternatives, such as methanol. In this chapter, the advances in constructing competitive multi-enzymatic systems for the reduction of CO2 to methanol are discussed. Different integrated methods are presented, aiming to address technological challenges, such as the cost effectiveness, need for material regeneration and reuse and improving product yields of the process. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

    Citation

    Io Antonopoulou, Ulrika Rova, Paul Christakopoulos. CO2 to Methanol: A Highly Efficient Enzyme Cascade. Methods in molecular biology (Clifton, N.J.). 2022;2487:317-344

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    PMID: 35687244

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