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    Herein, a facile fluorescent CRISPR-Cas12a-based sensing strategy is presented for prostate specific antigen (PSA), as a prostate cancer biomarker, with the assistance of a cruciform DNA nanostructure and PicoGreen (PG) as a fluorochrome. Highly sensitive recognition of PSA is one of the virtues of the proposed method which comes from the use of unique features of both CRISPR-Cas12a and DNA structure in the design of the aptasensor. The presence of PSA creates a cruciform DNA nanostructure in the sample which can be loaded by PG and make sharp fluorescence emission. While, when there is no PSA, the CRISPR-Cas12a digests sequences 1 and 3 as single-stranded DNAs, causing no DNA structure and a negligible fluorescence is detected after addition of PG. This aptasensor presents a sensitive recognition performance with detection limit of 4 pg/mL and a practical use for determination of PSA in serum samples. So, this analytical strategy introduces a convenient and highly sensitive approach for detection of disease biomarkers.Copyright © 2022 Elsevier B.V. All rights reserved.


    Seyed Mohammad Taghdisi, Mohammad Ramezani, Mona Alibolandi, Zahra Khademi, Mohammad Mahdi Hajihasani, Morteza Alinezhad Nameghi, Ali Khakshour Abdolabadi, Hoda Rahimi, Khalil Abnous, Noor Mohammad Danesh. A highly sensitive fluorescent aptasensor for detection of prostate specific antigen based on the integration of a DNA structure and CRISPR-Cas12a. Analytica chimica acta. 2022 Aug 01;1219:340031

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    PMID: 35715136

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