Polyvinyl alcohol can stabilize FITC conjugated recombinant annexin V for apoptotic cells detection.

Annexin V, a member of calcium-dependent phospholipid-binding proteins, selectively binds to the exposed phosphatidylserine, which can be used for in vitro apoptosis detection. Simultaneous staining of cells with annexin V-fluorescein isothiocyanate (FITC) and the non-vital dye propidium iodide (PI) enables detection of apoptotic and necrotic cells. Our study aimed to express, purify, and stabilize the recombinant annexin V. The recombinant annexin V was cloned and expressed in E. coli bacteria and was purified using Ni-IDA resin. The FITC conjugation was performed, and apoptosis detection of HaCaT cells by FITC-labeled annexin V was evaluated by flow cytometry. Then, the stability of FITC-labeled annexin in various conditions, including polyvinyl alcohol (PVA), glycerol, and trehalose, was evaluated. The results showed that annexin V was appropriately expressed and purified. After FITC conjugation, it could perfectly detect the cell death of HaCat cells in different apoptosis percentages. FITC-labeled annexin had more stability with PVA than glycerol and trehalose. It seems that PVA has an acceptable effect on FITC-labeled annexin V stability in concentrations lower than 1 mg mL-1, without interfering in fluorescent intensity. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.

Citation

Saeideh Sadat Shobeiri, Mojtaba Sankian. Polyvinyl alcohol can stabilize FITC conjugated recombinant annexin V for apoptotic cells detection. Protein and peptide letters. 2022 Jun 17

PMID: 35718962

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