Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus.

Pediatric acute myeloid leukemia (AML) is a rare and heterogeneous disease that remains the major cause of mortality in children with leukemia. To improve the outcome of pediatric AML we need to gain knowledge on the biological bases of this disease. NUP98-KDM5A (NK5A) fusion protein is present in a particular subgroup of young pediatric patients with poor outcome. We report the generation and characterization of human Embryonic Stem Cell (hESC) clonal lines with inducible expression of NK5A. Temporal control of NK5A expression during hematopoietic differentiation from hESC will be critical for elucidating its participation during the leukemogenic process. Copyright © 2022 Domingo-Reinés, Martínez-Navajas, Montes, Lamolda, Simón, Castaño, Ríos-Pelegrina, Lopez-Hidalgo, García del Moral, Marchal, Real and Ramos-Mejía.

Citation

Joan Domingo-Reinés, Gonzalo Martínez-Navajas, Rosa Montes, Mar Lamolda, Iris Simón, Julio Castaño, Rosa Ríos-Pelegrina, Javier Luis Lopez-Hidalgo, Raimundo García Del Moral, Juan A Marchal, Pedro J Real, Verónica Ramos-Mejía. Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus. Frontiers in cell and developmental biology. 2022;10:846092

PMID: 35721502

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