DAPI (4',6-diamidino-2-phenylindole) Stains Compact Amyloid Plaques.

DAPI is conventionally used as a nuclear stain for cells in culture or tissue. Here we demonstrate that it binds specifically to the β-sheet core of amyloid-β plaques but not diffuse amyloid-β at the plaque periphery. The specific DAPI induced blue fluorescence is much stronger than amyloid plaque autofluorescence. DAPI staining of fibrillar amyloid deposit may yield a misleading impression of damaged or dying cells. On the other hand, it provides a handy and low-cost means of staining compact amyloid plaques together with cell nuclei in double or triple immunofluorescent studies.

Citation

Rana Mabrouk, Maria Gotkiewicz, Tuomas Rauramaa, Heikki Tanila. DAPI (4',6-diamidino-2-phenylindole) Stains Compact Amyloid Plaques. Journal of Alzheimer's disease : JAD. 2022;88(3):949-955

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PMID: 35754268

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