A Plate Reader-Based Measurement of the Cellular ROS Production Using Dihydroethidium and MitoSOX.

Chih-Yao Chung, Michael R Duchen

Methods in molecular biology (Clifton, N.J.) 2022

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Intracellular reactive oxygen species (ROS) act as an important signaling transductor in cells, regulating almost every aspect of cell biology. Measurements of ROS production thus, offer links between oxidative stress and cell pathophysiology. Here, we describe a simple screening assay in intact adherent cells by fluorescence microplate readers, using dihydroethidium (DHE) and MitoSOX to measure cytosolic superoxide and mitochondrial superoxide production, respectively. This assay enables a quick and reliable assessment of ROS generation in a well-controlled environment. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Chih-Yao Chung, Michael R Duchen. A Plate Reader-Based Measurement of the Cellular ROS Production Using Dihydroethidium and MitoSOX. Methods in molecular biology (Clifton, N.J.). 2022;2497:333-337