In yeast, cardiolipin unsaturation level plays a key role in mitochondrial function and inner membrane integrity.

Luis Alberto Luévano-Martínez, Isabella Fernanda Dantas Pinto, Marcos Yukio Yoshinaga, Sayuri Miyamoto

Biochimica et biophysica acta. Bioenergetics 2022 Oct 01

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Cardiolipin is the signature phospholipid of the mitochondrial inner membrane. It participates in shaping the inner membrane as well as in modulating the activity of many membrane-bound proteins. The acyl chain composition of cardiolipin is finely tuned post-biosynthesis depending on the surrounding phospholipids to produce mature or unsaturated cardiolipin. However, experimental evidence showing that immature and mature cardiolipin are functionally equivalents for mitochondria poses doubts on the relevance of cardiolipin remodeling. In this work, we studied the role of cardiolipin acyl chain composition in mitochondrial bioenergetics, including a detailed bioenergetic profile of yeast mitochondria. Cardiolipin acyl chains were modified by genetic and nutritional manipulation. We found that both the bioenergetic efficiency and osmotic stability of mitochondria are dependent on the unsaturation level of cardiolipin acyl chains. It is proposed that cardiolipin remodeling and, consequently, mature cardiolipins play an important role in mitochondrial inner membrane integrity and functionality. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Luis Alberto Luévano-Martínez, Isabella Fernanda Dantas Pinto, Marcos Yukio Yoshinaga, Sayuri Miyamoto. In yeast, cardiolipin unsaturation level plays a key role in mitochondrial function and inner membrane integrity. Biochimica et biophysica acta. Bioenergetics. 2022 Oct 01;1863(7):148587