Determination of putrescine in brain tissue using gas chromatography-mass spectrometry.

T Noto, T Hasegawa, H Kamimura, J Nakao, H Hashimoto, T Nakajima

Analytical biochemistry 1987 Feb 1

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We used gas chromatography-mass spectrometry to assay putrescine in minute regions of single rat brains. Acid extraction, partial purification on Amberlite CG 120, and derivatization with pentafluoropropionic anhydride preceded the gas chromatography-mass spectrometry. A moving-needle solventless system and a direct inlet system were also used to increase sensitivity. Putrescine was measured accurately at the picomole level; the mean concentration of this polyamine in five regions of rat brain found by this method was 2.7-3.8 times higher than reported by other researchers.

Citation

T Noto, T Hasegawa, H Kamimura, J Nakao, H Hashimoto, T Nakajima. Determination of putrescine in brain tissue using gas chromatography-mass spectrometry. Analytical biochemistry. 1987 Feb 1;160(2):371-5