Effect of IL4 and IL10 on a human in vitro type 1 diabetes model.

Susanne E M Pfeiffer, Estefania Quesada-Masachs, Sara McArdle, Samuel Zilberman, Burcak Yesildag, Zbigniew Mikulski, Matthias von Herrath

Clinical immunology (Orlando, Fla.) 2022 Aug

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We defined the effect of the anti-inflammatory cytokines IL4 and IL10 on an in vitro model of human T1D. After preincubation with IL4 or IL10, human islet microtissues were co-cultured with PBMC and proinflammatory cytokines for a few hours or for multiple days to assess acute and chronic effects. This resulted in an immune attack with infiltration of T cells into the islet, a loss of beta cell endocrine function, and an upregulation of HLA-I on the beta cells. HLA-I upregulation was associated with infiltration of T cells and both HLA-I expression and infiltration were associated with impaired insulin secretion. Preincubation with IL4 or IL10 did not preserve beta cell function but decreased infiltration of T cells. Our data support the hypothesis that a loss of beta cell endocrine function mediates an increase in beta cell specific antigen presentation. IL4 and IL10 failed to preserve beta cell endocrine function. Copyright © 2022 Elsevier Inc. All rights reserved.

Citation

Susanne E M Pfeiffer, Estefania Quesada-Masachs, Sara McArdle, Samuel Zilberman, Burcak Yesildag, Zbigniew Mikulski, Matthias von Herrath. Effect of IL4 and IL10 on a human in vitro type 1 diabetes model. Clinical immunology (Orlando, Fla.). 2022 Aug;241:109076