Bioluminescent Tracking of Human Induced Pluripotent Stem Cells In Vitro and In Vivo.

Toshinobu Nishimura, Kouta Niizuma, Hiromitsu Nakauchi

Methods in molecular biology (Clifton, N.J.) 2022

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The discovery and development of induced pluripotent stem cells (iPSCs) opened a novel venue for disease modeling, drug discovery, and personalized medicine. Additionally, iPSCs have been utilized for a wide variety of research and clinical applications without immunological and ethical concerns that arise from using embryonic stem cells. Understanding the in vivo behavior of iPSCs, as well as their derivatives, requires the monitoring of their localization, proliferation, and viability after transplantation. Bioluminescence imaging (BLI) gives investigators a non-invasive and sensitive means for spatio-temporal tracking in vivo. For scientists working within the field of iPSCs, this protocol provides a walk-through on how to conduct in vitro and in vivo experiments with an iPSCs constitutively expressing luciferase. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Toshinobu Nishimura, Kouta Niizuma, Hiromitsu Nakauchi. Bioluminescent Tracking of Human Induced Pluripotent Stem Cells In Vitro and In Vivo. Methods in molecular biology (Clifton, N.J.). 2022;2524:291-297