MiR-192-5p Alleviated Fibrosis and Inflammatory Responses of Tendon Cells by Targeting NFAT5.

Fan Gong, Xiaoliang Li, Hanling Zhang, Jianke Wu, Guoxu Ma, Bowen Zhang, Jian Gao, Yi Ding, Yonglu Huang, Kun Xia, Suoli Cheng, Xuebing Zhou, Jiandang Shi, Fei Zhao

Computational and mathematical methods in medicine 2022

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To explore the effect of microRNA (miR)-192-5p on the inflammatory and fibrotic responses of tendon cells. Tendon cells were treated with transforming growth factor-β1 (TGF-β1). The expression of miR-192-5p and nuclear factor of activated T cells 5 (NFAT5) in tendon cells were detected by RT-qPCR. The expressions of inflammatory and fibrosis-related factors were detected by RT-qPCR and Western blot. MiR-192-5p binds to NFAT5 targeting by TargetScan and dual-luciferase reporter gene assay. The expression of the NFAT5 gene was detected by RT-qPCR and Western blot. Detection of apoptosis in tendon cells by flow cytometry. MiR-192-5p was downregulated in tendon cells, and the expression level gradually decreased with the prolong of TGF-β1 treatment. The expression of NFAT5 increased with the treatment time of TGF-β1. The expression of miR-192-5p decreased collagen III (COLIII), α smooth muscle actin (α-SMA), matrix metalloproteinase- (MMP-) 1, and MMP-8 expression, thereby inhibiting TGF-β1-induced fibrosis in tendon cells. The expression of miR-192-5p decreased the expression of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-1β, thereby alleviating TGF-β1-induced inflammatory response and reduce apoptosis in tendon cells. NFAT5 is a direct target of miR-192-5p in tendon cells. The upregulation of NFAT5 reversed the effect of miR-192-5p on the fibrotic activity and inflammatory response of TGF-β1-stimulated tendon cells. MiR-192-5p alleviates fibrosis and inflammatory responses of tendon cells by targeting NFAT5. Copyright © 2022 Fan Gong et al.

Citation

Fan Gong, Xiaoliang Li, Hanling Zhang, Jianke Wu, Guoxu Ma, Bowen Zhang, Jian Gao, Yi Ding, Yonglu Huang, Kun Xia, Suoli Cheng, Xuebing Zhou, Jiandang Shi, Fei Zhao. MiR-192-5p Alleviated Fibrosis and Inflammatory Responses of Tendon Cells by Targeting NFAT5. Computational and mathematical methods in medicine. 2022;2022:6481846