Polysaccharide from Echinacea purpurea plant ameliorates oxidative stress-induced liver injury by promoting Parkin-dependent autophagy.

Tingdong Yu, Yanan He, Haitao Chen, Xiaokai Lu, Huijing Ni, Yimin Ma, Yumei Chen, Chen Li, Run Cao, Liju Ma, Zhiyao Li, Yujie Lei, Xiaomao Luo, Chenhong Zheng

Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Sep

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Acetaminophen (APAP) overdose represents one of the most common drug-induced liver injuries (DILI) worldwide. Oxidative damage to the hepatocytes and their resultant autophagy are the key components in the APAP-induced DILI. Echinacea purpurea polysaccharide (EPPS), the component extracted from the root of Echinacea purpurea (L.) Moench, shows various biological functions including immunoregulation and antioxidant activity. This study aimed to elucidate the protective effect of EPPS against APAP-induced DILI and the underlying mechanisms. EPPS attenuates APAP overdose induced DILI in mice and ameliorates inflammation and oxidative stress in mice with APAP overdose-induced DILI. Furthermore, EPPS protected the hepatocytes against APAP-induced liver injury by suppressing apoptosis. EPPS ameliorates APAP-induced DILI via an autophagy-dependent mechanism in vivo and increases autophagy with a reduction in oxidative stress and inflammation in vitro. Parkin knockdown prevents the autophagic-dependent manner of EPPS effects in APAP-treated hepatocytes. EPPS exhibited a strong hepatoprotective effect against APAP-induced DILI and was correlated with reduction of autophagy-dependent oxidant response, inflammation, and apoptosis. Moreover, the findings indicated that EPPS exerts its hepatoprotective effect against APAP mainly via Parkin-dependent autophagy, and the use of EPPS can serve as a promising novel therapeutic strategy for APAP-induced DILI. Copyright © 2022. Published by Elsevier GmbH.

Citation

Tingdong Yu, Yanan He, Haitao Chen, Xiaokai Lu, Huijing Ni, Yimin Ma, Yumei Chen, Chen Li, Run Cao, Liju Ma, Zhiyao Li, Yujie Lei, Xiaomao Luo, Chenhong Zheng. Polysaccharide from Echinacea purpurea plant ameliorates oxidative stress-induced liver injury by promoting Parkin-dependent autophagy. Phytomedicine : international journal of phytotherapy and phytopharmacology. 2022 Sep;104:154311