Correlation Engine 2.0
Clear Search sequence regions

  • brain (1)
  • calcineurin (3)
  • calcium (4)
  • genes (1)
  • methyl (3)
  • mitogen (3)
  • neurons (3)
  • potassium (3)
  • protein kinases (2)
  • rats (1)
  • receptors d (2)
  • responses stimulus (2)
  • Sizes of these terms reflect their relevance to your search.

    Immediate early genes (IEGs) are transcribed in response to neuronal activity from sensory stimulation during multiple adaptive processes in the brain. The transcriptional profile of IEGs is indicative of the duration of neuronal activity, but its sensitivity to the strength of depolarization remains unknown. Also unknown is whether activity history of graded potential changes influence future neuronal activity. In this work with dissociated rat cortical neurons, we found that mild depolarization-mediated by elevated extracellular potassium (K+)-induces a wide array of rapid IEGs and transiently depresses transcriptional and signaling responses to a successive stimulus. This latter effect was independent of de novo transcription, translation, and signaling via calcineurin or mitogen-activated protein kinase. Furthermore, as measured by multiple electrode arrays and calcium imaging, mild depolarization acutely subdues subsequent spontaneous and bicuculline-evoked activity via calcium- and N-methyl-d-aspartate receptor-dependent mechanisms. Collectively, this work suggests that a recent history of graded potential changes acutely depress neuronal intrinsic properties and subsequent responses. Such effects may have several potential downstream implications, including reducing signal-to-noise ratio during synaptic plasticity processes. Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.


    Kira D A Rienecker, Robert G Poston, Joshua S Segales, Isabelle W Finholm, Morgan H Sono, Sorina J Munteanu, Mina Ghaninejad-Esfahani, Ayna Rejepova, Susana Tejeda-Garibay, Kevin Wickman, Ezequiel Marron Fernandez de Velasco, Stanley A Thayer, Ramendra N Saha. Mild membrane depolarization in neurons induces immediate early gene transcription and acutely subdues responses to a successive stimulus. The Journal of biological chemistry. 2022 Sep;298(9):102278

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 35863435

    View Full Text