The High-Salt Recovered Sequence-Sequencing (HRS-seq) Method: Exploring Genome Association with Nuclear Bodies.

Cosette Rebouissou, Marie-Odile Baudement, Thierry Forné

Methods in molecular biology (Clifton, N.J.) 2022

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Recent works indicate that, at specific loci, interactions of chromatin with membrane-less organelles self-assembled through mechanisms of phase separation, like nuclear bodies, are crucial to regulate genome functions, and in particular transcription. Here we describe the protocol of the high-salt recovered sequence sequencing method whose principle relies on high-throughput sequencing of genomic DNA trapped into large RNP complexes that are made insoluble by high-salt treatments. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Cosette Rebouissou, Marie-Odile Baudement, Thierry Forné. The High-Salt Recovered Sequence-Sequencing (HRS-seq) Method: Exploring Genome Association with Nuclear Bodies. Methods in molecular biology (Clifton, N.J.). 2022;2532:187-197