Expanding the versatility and scope of the oxime ligation: rapid bioconjugation to disulfide-rich peptides.

The oxime ligation is a valuable bioorthogonal conjugation reaction but with limited compatibility with disulfide-rich peptides/proteins and time-sensitive applications. Here we overcome these limitations by introducing a strategy that supports regiospecific control, oxidative folding, production of stable aminooxy-precursors for on-demand modification, and complete ligation within 5 min.

Citation

Anke Hering, Nayara Braga Emidio, Markus Muttenthaler. Expanding the versatility and scope of the oxime ligation: rapid bioconjugation to disulfide-rich peptides. Chemical communications (Cambridge, England). 2022 Aug 11;58(65):9100-9103

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PMID: 35880482

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