Characterisation of the RNA Virome of Nine Ochlerotatus Species in Finland.

Phuoc T Truong Nguyen, C Lorna Culverwell, Maija T Suvanto, Essi M Korhonen, Ruut Uusitalo, Olli Vapalahti, Teemu Smura, Eili Huhtamo

Viruses 2022 Jul 07

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RNA viromes of nine commonly encountered Ochlerotatus mosquito species collected around Finland in 2015 and 2017 were studied using next-generation sequencing. Mosquito homogenates were sequenced from 91 pools comprising 16-60 morphologically identified adult females of Oc. cantans, Oc. caspius, Oc. communis, Oc. diantaeus, Oc. excrucians, Oc. hexodontus, Oc. intrudens, Oc. pullatus and Oc. punctor/punctodes. In total 514 viral Reverse dependent RNA polymerase (RdRp) sequences of 159 virus species were recovered, belonging to 25 families or equivalent rank, as follows: Aliusviridae, Aspiviridae, Botybirnavirus, Chrysoviridae, Chuviridae, Endornaviridae, Flaviviridae, Iflaviridae, Negevirus, Partitiviridae, Permutotetraviridae, Phasmaviridae, Phenuiviridae, Picornaviridae, Qinviridae, Quenyavirus, Rhabdoviridae, Sedoreoviridae, Solemoviridae, Spinareoviridae, Togaviridae, Totiviridae, Virgaviridae, Xinmoviridae and Yueviridae. Of these, 147 are tentatively novel viruses. One sequence of Sindbis virus, which causes Pogosta disease in humans, was detected from Oc. communis from Pohjois-Karjala. This study greatly increases the number of mosquito-associated viruses known from Finland and presents the northern-most mosquito-associated viruses in Europe to date.

Citation

Phuoc T Truong Nguyen, C Lorna Culverwell, Maija T Suvanto, Essi M Korhonen, Ruut Uusitalo, Olli Vapalahti, Teemu Smura, Eili Huhtamo. Characterisation of the RNA Virome of Nine Ochlerotatus Species in Finland. Viruses. 2022 Jul 07;14(7)