Hypothetical bromodomain-containing protein 5 is required for the growth of Toxoplasma gondii.

Ying Zhang, Long Cheng, Hongyu Qiu, Tiancong Sun, Ruipeng Deng, Haiyan Gong, Rongsheng Mi, Yan Huang, Chunren Wang, Zhaoguo Chen

Veterinary parasitology 2022 Sep

filter terms:

Bromodomain (BRD) is a highly conserved structural module domain, found in various proteins, including chromatin-related proteins, nucleus acetyltransferases, and transcription-associated proteins. Toxoplasma gondii, a zoonotic protozoan, encodes at least 12 predicted BRD-containing proteins (BDPs). Here, we investigated the subcellular location and regulatory role of a hypothetical protein BDP that we named TgBDP5. The BRD of TgBDP5 did not contain the conserved Asn and Tyr residues required for acetyl-lysine recognition. TgBDP5 localized in the nucleus of the parasite and remained unchanged during parasite replication. Conditional ablation of TgBDP5 through an auxin-inducible degron-based knockdown strategy caused a growth defect in parasite replication. Depletion of TgBDP5 led to changes in the expression level of 179 genes, suggesting it as an important target for drugs acting against T. gondii. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Ying Zhang, Long Cheng, Hongyu Qiu, Tiancong Sun, Ruipeng Deng, Haiyan Gong, Rongsheng Mi, Yan Huang, Chunren Wang, Zhaoguo Chen. Hypothetical bromodomain-containing protein 5 is required for the growth of Toxoplasma gondii. Veterinary parasitology. 2022 Sep;309:109767