Guangwei Jiang, Mingxuan Qu, Lili Kong, Xiaoming Song, Shanhao Jiang
BioMed research international 2022Retinoblastoma (RB) is the commonest primary intraocular malignancy during childhood. Circular RNAs (circRNAs) act as regulators in RB development, and hsa_circ_E2F5 (circ_0084811 in this study) was found to be highly expressed in RB cells, so we wanted to identify its detailed molecular mechanism. The expression level of circ_0084811 in RB cells was tested by RT-qPCR and its effects on RB cells were evaluated through functional assays. The regulatory mechanism that circ_0084811 may exert in RB progression was testified through mechanism experiments. High circ_0084811 expression in RB cells facilitated cell proliferation but inhibited cell apoptosis. The enrichment of acetylation of histone 3 lysine 27 (H3K27ac) in circ_0084811 promoter induced circ_0084811 upregulation. Moreover, circ_0084811 regulated E2F transcription factor 5 (E2F5) expression via sponging microRNA-18a-5p (miR-18a-5p) and microRNA-18b-5p (miR-18b-5p). circ_0084811 modulated RB progression via the miR-18a-5p/miR-18b-5p/E2F5 axis. Copyright © 2022 Guangwei Jiang et al.
Guangwei Jiang, Mingxuan Qu, Lili Kong, Xiaoming Song, Shanhao Jiang. hsa_circ_0084811 Regulates Cell Proliferation and Apoptosis in Retinoblastoma through miR-18a-5p/miR-18b-5p/E2F5 Axis. BioMed research international. 2022;2022:6918396
PMID: 35909488
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