A sensitive and quantitative immunochromatographic assay for simultaneous detection of three stimulant laxatives in slimming food.

Sha Chen, Tian Guan, Zhenjie Qian, Qiqi Quan, Jin Wang, Xiangmei Li, Shaozong Wu, Yiyong Yan, Xiaojun Yao, Shiwei Zhang, Hongtao Lei

Food chemistry 2023 Jan 01

filter terms:

Bisacodyl, sodium picosulfate and their metabolite bis-(p-hydroxyphenyl)-pyridyl-2-methane (BHPM) are clinically used to treat constipation. In this study, with the rational hapten design, a broad-specific and highly sensitive monoclonal antibody (mAb) was obtained with half of inhibitory concentrations of 0.16, 0.18 and 0.65 ng/mL for bisacodyl, sodium picosulfate and BHPM, respectively. Based on this mAb, a rapid and sensitive lateral flow immunochromatographic assay toward bisacodyl, sodium picosulfate and BHPM in slimming foods was developed. This method can qualitatively and quantitatively screen three stimulant laxatives with cut-off values of 3-6 ng/mL by naked eye and quantitative detection limits of 0.14-0.41 ng/mL by reader. The acceptable recoveries of spiked samples (78.00 %-120.12 %) and consistent results with liquid chromatography with tandem mass spectrometry (LC-MS/MS) in real sample detection confirmed the accuracy of the method. The established method provides a technique for multiplex, sensitive, fast, and on-site detection of three stimulant laxatives in slimming food. Copyright © 2022 Elsevier Ltd. All rights reserved.

Citation

Sha Chen, Tian Guan, Zhenjie Qian, Qiqi Quan, Jin Wang, Xiangmei Li, Shaozong Wu, Yiyong Yan, Xiaojun Yao, Shiwei Zhang, Hongtao Lei. A sensitive and quantitative immunochromatographic assay for simultaneous detection of three stimulant laxatives in slimming food. Food chemistry. 2023 Jan 01;398:133861