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Forward genetic screens are invaluable in describing gene function. CRISPR has reinvigorated phenotypic screens in Toxoplasma - a model apicomplexan parasite. Two recent papers by Smith et al. and Li et al. take the next big leap in performing forward genetic screens in Toxoplasma by combining conditional gene regulation with CRISPR. Crown Copyright © 2022. Published by Elsevier Ltd. All rights reserved.


Aurelie Tsee Dawson, Christopher J Tonkin. A CRISPR upgrade unlocks Toxoplasma gene function. Trends in parasitology. 2022 Oct;38(10):826-828

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PMID: 35973902

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