MicroRNA-124-3p promotes apoptosis and autophagy of glioma cells by down-regulating CREBRF.

Huan Zeng, Mengyi Huang, Xin Gong

Neurological research 2022 Dec

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This research was performed to dissect the influence of microRNA (miR)-124-3p on the apoptosis and autophagy of glioma cells and clarify its specific mechanism. RT-PCR and western blot were utilized to determine miR-124-3p and CREBRF expression in U251 and T98 cells. After loss- and gain-of-function assays in U251 and T98 cells, glioma cell proliferation, autophagy, and apoptosis were measured by MTT assay, western blot, and flow cytometry, respectively. The relationship between miR-124-3p and CREBRF was examined by dual-luciferase reporter assay. The levels of AKT pathway-related proteins were detected by western blot. MiR-124-3p was lowly expressed and CREBRF was highly expressed in U251 and T98 cells. Overexpression of miR-124-3p or knockdown of CREBRF enhanced apoptosis and autophagy and diminished proliferation of glioma cells. MiR-124-3p negatively targeted CREBRF. MiR-124-3p up-regulation repressed proliferation and facilitated apoptosis and autophagy of glioma cells by diminishing CREBRF expression and blocking the AKT pathway. MiR-124-3p accelerates apoptosis and autophagy of glioma cells via CREBRF.

Citation

Huan Zeng, Mengyi Huang, Xin Gong. MicroRNA-124-3p promotes apoptosis and autophagy of glioma cells by down-regulating CREBRF. Neurological research. 2022 Dec;44(12):1094-1103