Frontiers in the enzymology of thiamin diphosphate-dependent enzymes.

Sabin Prajapati, Fabian Rabe von Pappenheim, Kai Tittmann

Current opinion in structural biology 2022 Oct

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Enzymes that use thiamin diphosphate (ThDP), the biologically active derivative of vitamin B1, as a cofactor play important roles in cellular metabolism in all domains of life. The analysis of ThDP enzymes in the past decades have provided a general framework for our understanding of enzyme catalysis of this protein family. In this review, we will discuss recent advances in the field that include the observation of "unusual" reactions and reaction intermediates that highlight the chemical versatility of the thiamin cofactor. Further topics cover the structural basis of cooperativity of ThDP enzymes, novel insights into the mechanism and structure of selected enzymes, and the discovery of "superassemblies" as reported, for example, acetohydroxy acid synthase. Finally, we summarize recent findings in the structural organisation and mode of action of 2-keto acid dehydrogenase multienzyme complexes and discuss future directions of this exciting research field. Copyright © 2022 Elsevier Ltd. All rights reserved.

Citation

Sabin Prajapati, Fabian Rabe von Pappenheim, Kai Tittmann. Frontiers in the enzymology of thiamin diphosphate-dependent enzymes. Current opinion in structural biology. 2022 Oct;76:102441