Isolation, Physicochemical Characterization, Labeling, and Biological Evaluation of Mannans and Glucans.

Michael D Kruppa, Douglas W Lowman, Harry E Ensley, Zuchao Ma, Bridget Graves, Jennifer Kintner, Jennifer V Hall, Tammy R Ozment, David L Williams

Methods in molecular biology (Clifton, N.J.) 2022

filter terms:

The cell wall contains mannans and glucans that are recognized by the host immune system. In this chapter, we will describe the methods to isolate mannans and glucans from the C. albicans cell wall. In addition, we describe how to determine purity, molecular size, and structure of the mannans and glucans. We also detail how to prepare the carbohydrates for in vitro, ex vivo, or in vivo use by describing endotoxin removal (depyrogenation), derivatization, and labeling and evaluation of bioactivity. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Michael D Kruppa, Douglas W Lowman, Harry E Ensley, Zuchao Ma, Bridget Graves, Jennifer Kintner, Jennifer V Hall, Tammy R Ozment, David L Williams. Isolation, Physicochemical Characterization, Labeling, and Biological Evaluation of Mannans and Glucans. Methods in molecular biology (Clifton, N.J.). 2022;2542:323-360