Adaptation of protein phosphatases in Oryza sativa and Cucumis sativus to microcystins.

Xudong Ma, Yanfang Gu, Chanjuan Liang

Environmental science and pollution research international 2023 Jan

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Microcystins (MCs) in irrigation water could inhibit crop growth and yield. Protein phosphatases (PPs) play an important role in regulating physiological mechanisms in plants to adapt abiotic stresses. To clarify the adaptation mechanism in plants to MCs stress, we compared PPs in rice and cucumber leaves by analyzing PPs total activity, protein phosphatase-2A (PP2A) activity and expression, as well as related growth and gas exchange parameters. After 7-day exposure of MCs (5 ~ 100 µg/L) and 7-day recovery without MCs, rice showed higher tolerance to MCs by analyzing dry weight and gas exchange parameters. Both crops may regulate PPs activity to adapt MCs stress by increasing the expression of genes encoding PPs. Among them, PP2A activity in two crops showed more sensitivity to MCs than total PPs activity. In addition, the higher expressions of PP2A catalytic and regulatory subunits and lower decrease PP2A activity were observed in rice leaves compared to cucumber. All results suggest that the expression levels of PP2A subunits could play a role in maintaining the activity of PP2A to regulating plant tolerance to MCs stress. © 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Citation

Xudong Ma, Yanfang Gu, Chanjuan Liang. Adaptation of protein phosphatases in Oryza sativa and Cucumis sativus to microcystins. Environmental science and pollution research international. 2023 Jan;30(3):7018-7029