Yue Liu, Xiaoxia Sun, Jincan Zhang, Feng Gao, Leilei Yu, Lina Dong, Gangli Zhang, Changxin Wu
FEMS microbiology letters 2022 Sep 07Although probiotics have been isolated from different sources, few were isolated from traditional Chinese medicine. The current study firstly isolate Pulsatilla Radix-utilizing Pediococcus pentosaceus PR-1 from human faeces. Subsequently, the tolerance of PR-1 to low pH, bile salts, simulated gastric juice and succus entericus, antioxidant activity, antimicrobial activity, cholesterol-assimilation and antibiotics susceptibility were investigated. After 2 h incubation at pH 2.0, over 80% of PR-1 survived. The cell viability of PR-1 at 2 h under 0.1% bile salt condition was 99.2%. The survival rate of PR-1 in gastric juice and succus entericus were 64.48% and 81.86, respectively. Cell-free supernatant of PR-1 culture also showed antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium. Besides, antioxidant activity of PR-1 CFS was significantly greater than cell pellet. PR-1 was shown resistant to kanamycin, streptomycin, vancomycin, and norfloxacin and able to lower cholesterol level to 72.5 ± 1.5%. In addition, PR-1 displayed γ-hemolysis and was non-pathogenic.© The Author(s) 2022. Published by Oxford University Press on behalf of FEMS.
Yue Liu, Xiaoxia Sun, Jincan Zhang, Feng Gao, Leilei Yu, Lina Dong, Gangli Zhang, Changxin Wu. Isolation and characterisation of Pulsatilla Radix-utilizing bacteria Pediococcus pentosaceus PR-1 from human faeces. FEMS microbiology letters. 2022 Sep 07
PMID: 36073496
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