Detailed Structural Analysis of the Immunoregulatory Polysaccharides from the Mycobacterium Bovis BCG.

Lan Luo, Xuemei Song, Xiao Chang, Sheng Huang, Yunxi Zhou, Shengmei Yang, Yan Zhu, Lanyan Zhang, Yongsheng Wu, Junyin Zhang, Zhipeng Zhou, Mingyi Wu

Molecules (Basel, Switzerland) 2022 Sep 03

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Bacillus Calmette-Guérin polysaccharide and nucleic acid (BCG-PSN), extracted from Mycobacterium bovis, is an immunoregulatory medicine commonly used in clinic. However, the structural characteristics and potential pharmacological efficacy of the polysaccharides from BCG-PSN remain unclear. Herein, two polysaccharides (BCG-1 and BCG-2) were purified and their structures were characterized. Monosaccharide composition analysis combined with methylation analysis and NMR data indicated that BCG-1 and BCG-2 were an α-D-(1→4)-mannan with (1→2)-linked branches, and an α-D-(1→4)-glucan with (1→6)-linked branches, respectively. Herein, the mannan from BCG-PSN was first reported. Bioactivity assays showed that BCG-1 and BCG-2 dose-dependently and potently increased the production of inflammatory mediators (NO, TNF-α, IL-6, IL-1β, and IL-10), as well as their mRNA expressions in RAW264.7 cells; both have similar or stronger effects compared with BCG-PSN injection. These data suggest that BCG-1 and BCG-2 are very likely the active ingredients of BCG-PSN.

Citation

Lan Luo, Xuemei Song, Xiao Chang, Sheng Huang, Yunxi Zhou, Shengmei Yang, Yan Zhu, Lanyan Zhang, Yongsheng Wu, Junyin Zhang, Zhipeng Zhou, Mingyi Wu. Detailed Structural Analysis of the Immunoregulatory Polysaccharides from the Mycobacterium Bovis BCG. Molecules (Basel, Switzerland). 2022 Sep 03;27(17)