Core-shell SERS nanotags-based western blot.

Western blot (WB) is the most commonly used scheme for protein identification in life science, but it still faces great challenges in the accurate quantitative detection of low-abundance proteins. Here, we proposed a novel surface-enhanced Raman scattering-based Western blot (SERS-WB) to solve this challenge. SERS nanotags were used as quantitative labels of proteins, which were composed of gold-silver core-shell nanoparticles, and Nile blue A (NBA) molecules were anchored on the interface of the core and shell. The results show that the SERS-WB possessed excellent sensitivity with detection limit of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein of 0.15 pg, as well as wide linear dynamic range (LDR) of 382 fg to 382 ng. In addition, the target protein on nitrocellulose (NC) membrane could be directly identified by colorimetric signal due to the aggregation effect of nanoparticles, which greatly simplifies the procedure. This as-proposed strategy will bring new thoughts to technological innovation of WB. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Bing Liu, Hanyu Tang, Qian Liu, Wenwen Wang, Haitao Li, Shiya Zheng, Fei Sun, Xiangwei Zhao. Core-shell SERS nanotags-based western blot. Talanta. 2023 Feb 01;253:123888

Mesh Tags

PMID: 36087412

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