Microbial transformation of betulonic acid by Circinella muscae CGMCC 3.2695 and anti-neuroinflammatory activity of the products.

Microbial transformation of betulonic acid with Circinella muscae CGMCC 3.2695 yielded nine undescribed metabolites and eight known compounds. The structures of the metabolites were established based on extensive NMR and HR-ESI-MS data analyses. It is shown that C. muscae could catalyze the regioselective hydroxylation at C-2, C-7, C-15, C-16, C-21, and C-30 along with carbonylation at C-2 and C-21. Furthermore, potential anti-neuroinflammatory activities of the obtained compounds in NO production were tested in lipopolysaccharides-induced BV-2 cells. Some of the metabolites exhibited pronounced inhibitory activities with IC50 values of 4.27-16.68 μM. Copyright © 2022 Elsevier Ltd. All rights reserved.

Citation

Youjia Lu, Yifei Tang, Yanni Wu, Xueying Zhang, Ying Yi, Wenli Wang, Andong Wang, Min Yang, Boyi Fan, Guangtong Chen. Microbial transformation of betulonic acid by Circinella muscae CGMCC 3.2695 and anti-neuroinflammatory activity of the products. Phytochemistry. 2022 Sep 12;204:113431

PMID: 36100092

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