miR-181a-5p is a potential candidate epigenetic biomarker in Multiple Sclerosis.

Multiple Sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) characterized by demyelination and axonal degeneration. Abnormal expression of microRNAs (miRNA) plays an important role in MS pathology. In this cohort study, differential expression of the four miRNAs (hsa-miR-155-5p, hsa-miR-9-5p, hsa-miR-181a-5p, and hsa-miR-125b-5p) were investigated in 69 individuals, including 39 MS patients (relapsing-remitting MS (RRMS), n = 27; secondary progressive MS (SPMS), n = 12) and 30 healthy controls. In silico analyzes revealed possible genes and pathways specific to miRNAs. Peripheral blood miRNA expressions were detected by quantitative Real-Time PCR (qPCR). hsa-miR-181a-5p was downregulated and associated with increased MS risk (P = 0.012). The other three miRNAs were upregulated and not associated with MS (P < 0.05). The area under the curve (AUC) is 0.779. In silico analyzes showed that hsa-miR-181a-5p may participate in MS pathology by targeting MAP2K1, CREB1, ATXN1, and ATXN3 genes in inflammation and neurodegeneration pathways. The circulatory hsa-miR-181a-5p can regulate target genes, reversing the mechanisms involved in MS pathologies such as protein uptake and processing, cell proliferation and survival, inflammation, and neurodegeneration. Thus, this miRNA could be used as an epigenomic-guided diagnostic tool and therapeutic.

Citation

Tuba Gökdoğan Edgünlü, Şenay Görücü Yılmaz, Ufuk Emre, Bahar Taşdelen, Oktay Kuru, Gülnihal Kutlu, Mehmet Emin Erdal. miR-181a-5p is a potential candidate epigenetic biomarker in Multiple Sclerosis. Genome. 2022 Sep 14

PMID: 36103723

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