Negative supercoils regulate meiotic crossover patterns in budding yeast.

Taicong Tan, Yingjin Tan, Ying Wang, Xiao Yang, Binyuan Zhai, Shuxian Zhang, Xuan Yang, Hui Nie, Jinmin Gao, Jun Zhou, Liangran Zhang, Shunxin Wang

Nucleic acids research 2022 Oct 14

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Interference exists ubiquitously in many biological processes. Crossover interference patterns meiotic crossovers, which are required for faithful chromosome segregation and evolutionary adaption. However, what the interference signal is and how it is generated and regulated is unknown. We show that yeast top2 alleles which cannot bind or cleave DNA accumulate a higher level of negative supercoils and show weaker interference. However, top2 alleles which cannot religate the cleaved DNA or release the religated DNA accumulate less negative supercoils and show stronger interference. Moreover, the level of negative supercoils is negatively correlated with crossover interference strength. Furthermore, negative supercoils preferentially enrich at crossover-associated Zip3 regions before the formation of meiotic DNA double-strand breaks, and regions with more negative supercoils tend to have more Zip3. Additionally, the strength of crossover interference and homeostasis change coordinately in mutants. These findings suggest that the accumulation and relief of negative supercoils pattern meiotic crossovers. © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

Citation

Taicong Tan, Yingjin Tan, Ying Wang, Xiao Yang, Binyuan Zhai, Shuxian Zhang, Xuan Yang, Hui Nie, Jinmin Gao, Jun Zhou, Liangran Zhang, Shunxin Wang. Negative supercoils regulate meiotic crossover patterns in budding yeast. Nucleic acids research. 2022 Oct 14;50(18):10418-10435