In vivo high-content imaging and regression analysis reveal non-cell autonomous functions of Shroom3 during neural tube closure.

Austin T Baldwin, Juliana H Kim, John B Wallingford

Developmental biology 2022 Nov

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During neural tube closure, neural ectoderm cells constrict their apical surfaces to bend and fold the tissue into a tube that will become the central nervous system. Recent data from mice and humans with neural tube defects suggest that key genes required for neural tube closure can exert non-cell autonomous effects on cell behavior, but the nature of these effects remains obscure. Here, we coupled tissue-scale, high-resolution time-lapse imaging of the closing neural tube of Xenopus to multivariate regression modeling, and we show that medial actin accumulation drives apical constriction non-autonomously in neighborhoods of cells, rather than solely in individual cells. To further explore this effect, we examined mosaic crispant embryos and identified both autonomous and non-autonomous effects of the apical constriction protein Shroom3. Copyright © 2022 Elsevier Inc. All rights reserved.

Citation

Austin T Baldwin, Juliana H Kim, John B Wallingford. In vivo high-content imaging and regression analysis reveal non-cell autonomous functions of Shroom3 during neural tube closure. Developmental biology. 2022 Nov;491:105-112