ANTIPLASMODIAL ACTIVITIES OF THE STEM BARK EXTRACT OF ARTOCARPUS ALTILIS FORSBERG.

The potential of Artocarpus altilis stem bark as a safe antimalarial agent, and the identification of its antimalarial constituents was explored. The air-dried stem bark was extracted with 70% ethanol, filtered and concentrated in vacuo to obtain the extract (EE). The extract was successively partitioned to give n-hexane (AAH), dichloromethane (AAD), ethyl acetate (AAE) n-butanol (AAB) and aqueous (AAQ) fractions respectively after determining the acute toxicity using Lorke's method. These were each evaluated for chemosuppressive antimalarial activities (0-200mg/kg) against chloroquine-sensitive Plasmodium berghei-berghei-infected albino mice. Normal saline and chloroquine, 10 mg/kg were negative and positive control respectively.The survival times and percentage survivors of the mice in both experiments were determined after observation for twenty-eight days post-drug administration. The five (5) column chromatographic (CC) fractions, AAH1, AAH2, AAH3, AAH4 and AAH5 obtained from the most active AAH, were also evaluated for antimalarial activities (0-50mg/kg). Further column purification and repeated PTLC of AAH5 yielded three bands, which were finally subjected to GC-MS analysis. EE gave ED50 and LD50 values of 227.17and >5000 mg/kg while its partitioned fractions gave ED50 values as follows: AAH, 79.14; AAD, 215.59; AAE, 160.46, AAB, .42; and AAQ, 90.85 mg/kg respectively. The primary CC fractions also gave ED 50 values as follows: AAH1 21.95; AAH2, 26.96; AAH3, 21.30; AAH4, 20.92 and AAH5, 20.75 mg/kg respectively to identify AAH5 as the putative fraction. GC-MS analysis revealed eleven major compounds (1-11) in the three PTLC bands as the antiplasmodial constituents of the plant. The stem bark of A. altilis is a potential agent in malaria control which is safe for oral use. Copyright: © 2022 Afr. J. Infect. Diseases.

Citation

Aladesanmi, Adetunji Joseph, Odiba, Ogu Emmanuel, Odediran, Samuel Akintunde, Oriola, Ayodeji Olubunmi. ANTIPLASMODIAL ACTIVITIES OF THE STEM BARK EXTRACT OF ARTOCARPUS ALTILIS FORSBERG. African journal of infectious diseases. 2022;16(2 Suppl):33-45

PMID: 36124325

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