Live Fluorescence Visualization of Cellulose and Pectin in Plant Cell Walls.

Youssef Chebli, Anja Geitmann

Methods in molecular biology (Clifton, N.J.) 2023

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The plant cell wall comprises various types of macromolecules whose abundance and spatial distribution change dynamically and are crucial for plant architecture. High-resolution live cell imaging of plant cell wall components is, therefore, a powerful tool for plant cell biology and plant developmental biology. To acquire suitable data, the experimental setup for staining and imaging of non-fixed samples must be straightforward and avoid creating stress-induced artifacts. We present a detailed sample preparation and live image acquisition protocol for fluorescence visualization of cell wall components using commercially available probes and stains. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Youssef Chebli, Anja Geitmann. Live Fluorescence Visualization of Cellulose and Pectin in Plant Cell Walls. Methods in molecular biology (Clifton, N.J.). 2023;2566:269-279