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    Investigating at transmission electron microscopy the intracellular trafficking of hyaluronic acid-based nanoparticles remains a challenge due to their intrinsic weak electron density. Here we describe a simple protocol to stain hyaluronic acid that allows visualization of hyaluronic acid-based nanoparticles inside cells at both light and electron microscopy. By applying the critical-electrolyte-concentration Alcian blue method, these nanoparticles were observed as blue dots at bright-field microscopy or filled with fine electron dense precipitates at transmission electron microscopy. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.


    Mathieu Repellin, Flavia Carton, Giovanna Lollo, Manuela Malatesta. Alcian Blue Staining to Visualize Intracellular Hyaluronic Acid-Based Nanoparticles. Methods in molecular biology (Clifton, N.J.). 2023;2566:313-320

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    PMID: 36152262

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