Expression of O-glycosylated oncofetal fibronectin in alternatively activated human macrophages.

Macrophage (Mϕ) polarization is an essential phenomenon for the maintenance of homeostasis and tissue repair, and represents the event by which Mϕ reach divergent functional phenotypes as a result to specific stimuli and/or microenvironmental signals. Mϕ can be polarized into two main phenotypes, M1 or classically activated and M2 or alternatively activated. These two categories diverge in many aspects, such as secreted cytokines, markers of cell surface, and biological functions. Over the last 10 years, many potential markers have been proposed for both M1 and M2 human Mϕ. However, there is scarce information regarding the glycophenotype adopted by these cells. Here, we show that M2- but not M1-polarized Mϕ expresses high levels of an unusual glycoform of fibronectin (FN), named O-glycosylated oncofetal FN (onf-FN), found in fetal/cancer cells, but not in healthy tissues. The onf-FN expression was confirmed in vitro by Western blot and real-time RT-qPCR in primary and cell line monocyte-derived Mϕ. onf-FN was induced by IL-4 and IL-13, but not by pro-inflammatory stimuli (LPS and INF-γ). RNA and protein analysis clearly demonstrated that it is specifically associated with the M2 polarization. In conclusion, we show by the first time that O-glycosylated onf-FN is expressed by M2-polarized Mϕ. © 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Marcos Andre Rodrigues da Costa Santos, Jhenifer Santos Dos Reis, Carlos Antonio do Nascimento Santos, Kelli Monteiro da Costa, Pedro Marçal Barcelos, Karen Queiroz de Oliveira Francisco, Pedro Antônio Guimarães Notaroberto Barbosa, Emanuelle Damasceno Souza da Silva, Celio Geraldo Freire-de-Lima, Alexandre Morrot, Debora Decote-Ricardo, Israel Diniz-Lima, Jose Osvaldo Previato, Lucia Mendonca-Previato, Leonardo Marques da Fonseca, Leonardo Freire-de-Lima. Expression of O-glycosylated oncofetal fibronectin in alternatively activated human macrophages. Immunologic research. 2023 Feb;71(1):92-104

Mesh Tags

Substances

PMID: 36197587

search → result