Characterizing Fluorescence Properties of Turn-on RNA Aptamers.

Robert J Trachman, Katie A Link, Jay R Knutson, Adrian R Ferré-D'Amaré

Methods in molecular biology (Clifton, N.J.) 2023

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Fluorescent RNA aptamers are tools for studying RNA localization and interactions in vivo. The photophysical properties of these in vitro selected RNAs should be characterized prior to cellular imaging experiments. Here, we describe the process of determining the fluorophore affinity, fluorescence enhancement, and fluorescence lifetime(s) of the Mango-III fluorescence turn-on aptamer. Parameters determined through these protocols will aid in establishing conditions for live-cell imaging. © 2023. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.

Citation

Robert J Trachman, Katie A Link, Jay R Knutson, Adrian R Ferré-D'Amaré. Characterizing Fluorescence Properties of Turn-on RNA Aptamers. Methods in molecular biology (Clifton, N.J.). 2023;2568:25-36