DNA nanostructure-assisted detection of carcinoembryonic antigen with a solid-state nanopore.

Rong Tian, Ting Weng, Shanchuan Chen, Ji Wu, Bohua Yin, Wenhao Ma, Liyuan Liang, Wanyi Xie, Yunjiao Wang, Xiaoqing Zeng, Yajie Yin, Deqiang Wang

Bioelectrochemistry (Amsterdam, Netherlands) 2023 Feb

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In this paper, a novel detection technique for tumor marker carcinoembryonic antigen (CEA) has been developed by using a solid-state nanopore as a tool. The system utilizes the specific affinity between aptamer-modified magnetic Fe3O4 and CEA, rather than directly detecting the translocation of CEA through the nanopore. The aptamer-modified magnetic Fe3O4 was hybridized with tetrahedral DNA nanostructures (TDNs), and TDNs were released after CEA was added. We investigate the translocation behavior of individual TDNs through solid-state nanopores. The frequency of the blockage signals for TDNs is recorded for indirect detection of CEA. We realized the detection of CEA with a concentration as low as 0.1 nM and proved the specificity of the interaction between the aptamer. In addition, our designed nanopore sensing strategy can detect CEA in real samples. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Rong Tian, Ting Weng, Shanchuan Chen, Ji Wu, Bohua Yin, Wenhao Ma, Liyuan Liang, Wanyi Xie, Yunjiao Wang, Xiaoqing Zeng, Yajie Yin, Deqiang Wang. DNA nanostructure-assisted detection of carcinoembryonic antigen with a solid-state nanopore. Bioelectrochemistry (Amsterdam, Netherlands). 2023 Feb;149:108284