Photoswitchable Microgels for Dynamic Macrophage Modulation.

Dynamic manipulation of supramolecular self-assembled structures is achieved irreversibly or under non-physiological conditions, thereby limiting their biomedical, environmental, and catalysis applicability. In this study, microgels composed of azobenzene derivatives stacked via π-cation and π-π interactions are developed that are electrostatically stabilized with Arg-Gly-Asp (RGD)-bearing anionic polymers. Lateral swelling of RGD-bearing microgels occurs via cis-azobenzene formation mediated by near-infrared-light-upconverted ultraviolet light, which disrupts intermolecular interactions on the visible-light-absorbing upconversion-nanoparticle-coated materials. Real-time imaging and molecular dynamics simulations demonstrate the deswelling of RGD-bearing microgels via visible-light-mediated trans-azobenzene formation. Near-infrared light can induce in situ swelling of RGD-bearing microgels to increase RGD availability and trigger release of loaded interleukin-4, which facilitates the adhesion structure assembly linked with pro-regenerative polarization of host macrophages. In contrast, visible light can induce deswelling of RGD-bearing microgels to decrease RGD availability that suppresses macrophage adhesion that yields pro-inflammatory polarization. These microgels exhibit high stability and non-toxicity. Versatile use of ligands and protein delivery can offer cytocompatible and photoswitchable manipulability of diverse host cells. © 2022 Wiley-VCH GmbH.

Citation

Yuri Kim, Ramar Thangam, Jounghyun Yoo, Jeongyun Heo, Jung Yeon Park, Nayeon Kang, Sungkyu Lee, Jiwon Yoon, Kwang Rok Mun, Misun Kang, Sunhong Min, Seong Yeol Kim, Subin Son, Jihwan Kim, Hyunsik Hong, Gunhyu Bae, Kanghyeon Kim, Sanghyeok Lee, Letao Yang, Ja Yeon Lee, Jinjoo Kim, Steve Park, Dong-Hyun Kim, Ki-Bum Lee, Woo Young Jang, Bong Hoon Kim, Ramasamy Paulmurugan, Seung-Woo Cho, Hyun-Cheol Song, Seok Ju Kang, Wujin Sun, Yangzhi Zhu, Junmin Lee, Han-Jun Kim, Ho Seong Jang, Jong Seung Kim, Ali Khademhosseini, Yongju Kim, Sehoon Kim, Heemin Kang. Photoswitchable Microgels for Dynamic Macrophage Modulation. Advanced materials (Deerfield Beach, Fla.). 2022 Dec;34(49):e2205498

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PMID: 36268986

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