Stability of Hemoglobin Constant Spring Identified by Capillary Electrophoresis.

Jidapa Jaitheang, Amornchai Suksusut, Rung Settapiboon, Supaporn Amornsiriwat, Pranee Sutcharitchan, Noppacharn Uaprasert, Ponlapat Rojnuckarin

Laboratory medicine 2023 May 02

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Hemoglobin Constant Spring (HbCS) is often missed by routine hemoglobin analysis. The aim of this research was to study HbCS stability as identified by capillary electrophoresis (CE) to determine the specimen storage time limit. The EDTA blood of 29 HbCS samples were kept at 4°C and analyzed every workday until CE could not detect HbCS or until 7 weeks after blood collection. The genotypes were confirmed by multiplex polymerase chain reaction. The median subject age was 27 years and 10 subjects were male. The HbCS levels were stable during the first 7 days but became undetectable in 5 cases (17.2%) after 1 week. All of them were heterozygous HbCS. Longer detection times were correlated with the higher baseline HbCS levels, with a correlation coefficient of 0.582 (P ≤ 0.001). Routine hemoglobin typing and quantitation should be performed within 1 week after blood collection to detect low HbCS levels, especially in heterozygous HbCS. © The Author(s) 2022. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Citation

Jidapa Jaitheang, Amornchai Suksusut, Rung Settapiboon, Supaporn Amornsiriwat, Pranee Sutcharitchan, Noppacharn Uaprasert, Ponlapat Rojnuckarin. Stability of Hemoglobin Constant Spring Identified by Capillary Electrophoresis. Laboratory medicine. 2023 May 02;54(3):e91-e94