Genetics and Gene Editing Methods in Xenopus laevis and Xenopus tropicalis.

Matthew Guille, Robert Grainger

Cold Spring Harbor protocols 2023 Jun 01

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Our understanding of biological systems has for many years been heavily influenced by experimental approaches that exploit genetic methods. These include gain-of-function experiments that overexpress transgenes or ectopically express injected RNA and loss-of-function experiments that knock out genes or knock down RNAs. Here, we review how these methods have been applied in Xenopus frogs and introduce a variety of protocols for genetic manipulation of Xenopus laevis and Xenopus tropicalis. © 2023 Cold Spring Harbor Laboratory Press.

Citation

Matthew Guille, Robert Grainger. Genetics and Gene Editing Methods in Xenopus laevis and Xenopus tropicalis. Cold Spring Harbor protocols. 2023 Jun 01;2023(6):pdb.top107045