86 novel HLA-E alleles discovered through full-gene sequencing of 6227 hematopoietic cell transplant patients and unrelated donors.

Jonathan A M Lucas, Xenia Georgiou, Michael A Cooper, James Robinson, Steven G E Marsh, Neema P Mayor

HLA 2023 Jan

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Until recently the number of alleles of the nonclassical HLA class I gene HLA-E documented in the IPD-IMGT/HLA Database was small and as a result, the gene was often not considered to be notably polymorphic. Here, we describe our work in identifying and submitting 86 novel HLA-E alleles after full-gene single-molecule real-time (SMRT) DNA sequencing of 6227 DNA samples. These samples were comprised of 2468 patients undergoing hematopoietic cell transplantation and 3759 unrelated potential donors. A total of 111 unique HLA-E alleles were detected in this cohort. The majority of novel alleles (79.1%) contained polymorphisms in intronic regions, highlighting the significant undiscovered variation present in the noncoding regions of the HLA-E gene. © 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Citation

Jonathan A M Lucas, Xenia Georgiou, Michael A Cooper, James Robinson, Steven G E Marsh, Neema P Mayor. 86 novel HLA-E alleles discovered through full-gene sequencing of 6227 hematopoietic cell transplant patients and unrelated donors. HLA. 2023 Jan;101(1):34-41