Merkel cell polyomavirus (MCPyV) DNA prevalence in Brazilian blood donors.
Victoria Simionatto Zucherato, Perícles Natan Mendes da Costa, Marta Giovanetti, Luciana Maria Fontanari Krause, Daiani Cristina Cilião Alves, Renata Maria Alencar Moreira, Barbara Maciel Sidou Pimentel, Rodrigo Haddad, Hellen Tayaná Bitencourt, Massimo Ciccozzi, Luiz Carlos Júnior Alcantara, Simone Kashima, Dimas Tadeu Covas, Svetoslav Nanev Slavov
Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine 2023 FebMerkel cell polyomavirus (MCPyV) is an oncogenic virus that has been etiologically linked to Merkel cell carcinoma. Low levels of MCPyV DNA have been detected in blood donors with unclear impact on transfusion. The prevalence of MCPyV DNA in Brazilian blood donors is unclear. Therefore, the objective of this study was to evaluate the MCPyV DNA prevalence among Brazilian blood donors. We examined the presence of MCPyV DNA by real-time PCR (qPCR) in a total of 450 serum samples obtained from blood donors from three Brazilian regions (North, Central-West and South). The overall estimated MCPyV DNA prevalence was 1.1% (CI = 95%, 0.16-2.06%). Divided by region, in North Brazil (city of Macapa, state of Amapá) and South Brazil (city of Santa Maria, state of Rio Grande do Sul), the MCPyV prevalence was the same: 1.33% (CI = 95%, range 0.0-3.14%). In Central-West Brazil (city of Brasilia), the MCPyV prevalence was 0.6% (CI = 95%, 0.0-1.96%). All MCPyV positive samples showed a high cycle threshold (median Ct = 35.5), most probably related to the low viral load. More studies are necessary to unveil the impact of this oncogenic virus on transfusion medicine and if such exists, especially in regards of its infectivity and transmission potential. Copyright © 2022 Société française de transfusion sanguine (SFTS). Published by Elsevier Masson SAS. All rights reserved.
Citation
Victoria Simionatto Zucherato, Perícles Natan Mendes da Costa, Marta Giovanetti, Luciana Maria Fontanari Krause, Daiani Cristina Cilião Alves, Renata Maria Alencar Moreira, Barbara Maciel Sidou Pimentel, Rodrigo Haddad, Hellen Tayaná Bitencourt, Massimo Ciccozzi, Luiz Carlos Júnior Alcantara, Simone Kashima, Dimas Tadeu Covas, Svetoslav Nanev Slavov. Merkel cell polyomavirus (MCPyV) DNA prevalence in Brazilian blood donors. Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine. 2023 Feb;30(1):143-146
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PMID: 36306977
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