Puerol and pueroside derivatives from Pueraria lobata and their anti-inflammatory activity.

Ying-Jie Sun, Shi-Jie Cao, Feng-Ni Liang, Jin-Yan Li, Xiang-Yu Zhang, Wei Li, Li-Qin Ding, Feng Qiu

Phytochemistry 2023 Jan

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Five undescribed puerols and puerosides and three known analogues were obtained from the roots of Pueraria lobata. Their structures were determined by comprehensive analysis of spectroscopic data and chemical methods. Since puerol D and puerol C were racemic compounds, resolved into their enantiomers, and their absolute configurations were determined by experimental and calculated ECD spectra. Six of the isolates were evaluated for their inhibitory activities on NO generation and the expression of inflammatory factors in the LPS-stimulated RAW 264.7 macrophage cells. The results showed that (S)-puerol C, (R)-puerol C, isokuzubutenolide A and kuzubutenolide A significantly decreased the NO production (IC50 values in the range of 16.87-39.95 μM). Meanwhile, (S)-puerol C, isokuzubutenolide A and kuzubutenolide A also reduced the mRNA expression of inflammatory factors (TNF-α, IL-1β and IL-6). Copyright © 2022 Elsevier Ltd. All rights reserved.

Citation

Ying-Jie Sun, Shi-Jie Cao, Feng-Ni Liang, Jin-Yan Li, Xiang-Yu Zhang, Wei Li, Li-Qin Ding, Feng Qiu. Puerol and pueroside derivatives from Pueraria lobata and their anti-inflammatory activity. Phytochemistry. 2023 Jan;205:113507