Sometimes faster can be better: Microneedling IPG strips enables higher throughput for integrative top-down proteomics.

Passive rehydration of immobilized pH gradient (IPG) strips for two-dimensional gel electrophoresis (2DE) has, to our knowledge, never been quantitatively evaluated to determine an ideal rehydration time. Seeking to increase throughput without sacrificing analytical rigor, we report that a substantially shorter rehydration time is accomplished when surface area of IPG strips is increased via microneedling. Rehydration for 4 h, post microneedling, provides comparable results to overnight rehydration in final analyses by 2DE, while also shortening the overall protocol by 1 day. © 2022 Wiley-VCH GmbH.

Citation

Katrina Carbonara, Jens R Coorssen. Sometimes faster can be better: Microneedling IPG strips enables higher throughput for integrative top-down proteomics. Proteomics. 2023 Jan;23(2):e2200307

Mesh Tags

PMID: 36349823

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