Nanofiber-coated, tacrolimus-eluting sutures inhibit post-operative neointimal hyperplasia in rats.

Kunal S Parikh, Aditya Josyula, Takahiro Inoue, Takuma Fukunishi, Huaitao Zhang, Revaz Omiadze, Richard Shi, Youseph Yazdi, Justin Hanes, Laura M Ensign, Narutoshi Hibino

Journal of controlled release : official journal of the Controlled Release Society 2023 Jan

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Post-operative complications of vascular anastomosis procedures remain a significant clinical challenge and health burden globally. Each year, millions of anastomosis procedures connect arteries and/or veins in vascular bypass, vascular access, organ transplant, and reconstructive surgeries, generally via suturing. Dysfunction of these anastomoses, primarily due to neointimal hyperplasia and the resulting narrowing of the vessel lumen, results in failure rates of up to 50% and billions of dollars in costs to the healthcare system. Non-absorbable sutures are the gold standard for vessel anastomosis; however, damage from the surgical procedure and closure itself causes an inflammatory cascade that leads to neointimal hyperplasia at the anastomosis site. Here, we demonstrate the development of a novel, scalable manufacturing system for fabrication of high strength sutures with nanofiber-based coatings composed of generally regarded as safe (GRAS) polymers and either sirolimus, tacrolimus, everolimus, or pimecrolimus. These sutures provided sufficient tensile strength for maintenance of the vascular anastomosis and sustained drug delivery at the site of the anastomosis. Tacrolimus-eluting sutures provided a significant reduction in neointimal hyperplasia in rats over a period of 14 days with similar vessel endothelialization in comparison to conventional nylon sutures. In contrast, systemically delivered tacrolimus caused significant weight loss and mortality due to toxicity. Thus, drug-eluting sutures provide a promising platform to improve the outcomes of vascular interventions without modifying the clinical workflow and without the risks associated with systemic drug delivery. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Kunal S Parikh, Aditya Josyula, Takahiro Inoue, Takuma Fukunishi, Huaitao Zhang, Revaz Omiadze, Richard Shi, Youseph Yazdi, Justin Hanes, Laura M Ensign, Narutoshi Hibino. Nanofiber-coated, tacrolimus-eluting sutures inhibit post-operative neointimal hyperplasia in rats. Journal of controlled release : official journal of the Controlled Release Society. 2023 Jan;353:96-104