Study on the spatial distribution of coumarins in Angelica dahurica root by MALDI-TOF-MSI.

Hui Gao, Qian Li

Phytochemical analysis : PCA 2023 Jan

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The main chemical components of Angelica dahurica (Hoffm.) Benth. & Hook.f. ex Franch. & Sav. are coumarins and volatile oils, and coumarins are regarded as the representative constituents with various pharmacological effects. Based on matrix-assisted laser desorption/ionization time of flight mass spectrometry imaging (MALDI-TOF-MSI), a method for spatial distribution analysis of coumarins in primary root and lateral root of A. dahurica was established. Also, spatial visualization of coumarins in the roots of A. dahurica was realized. α-Cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid, and 9-aminoacridine were used as matrices. MALDI-TOF-MSI was employed to analyze the standards of imperatorin, oxypeucedanin, and osthole. Based on the higher sensitivity and repeatability of MALDI-TOF-MSI, the CHCA matrix was selected. The matrix was used for MALDI-TOF-MSI in positive mode to analyze the distribution of coumarins in primary root and lateral root of A. dahurica. In total, 37 coumarins were detected in primary root and 36 coumarins were detected in lateral root by MALDI-TOF-MSI. The results showed that the coumarin content in primary root was higher than that in lateral root. Coumarins in primary root of A. dahurica were concentrated in the periderm, cortex, and phloem, whereas coumarins in lateral roots were concentrated in the phloem. The coumarins in primary root and lateral root of A. dahurica were directly analyzed without extraction and isolation, and the spatial distribution of coumarins was comprehensively visualized for the first time by MALDI-TOF-MSI, which provided a basis for distinguishing primary root and lateral root. © 2022 John Wiley & Sons Ltd.

Citation

Hui Gao, Qian Li. Study on the spatial distribution of coumarins in Angelica dahurica root by MALDI-TOF-MSI. Phytochemical analysis : PCA. 2023 Jan;34(1):139-148