Basophil Activation Test Shows Poor Sensitivity in Immediate Amoxicillin Allergy.

Kevin Heremans, Alessandro Toscano, Jessy Elst, Athina L Van Gasse, Christel Mertens, Michiel Beyens, Marie-Line M van der Poorten, Margo M Hagendorens, Didier G Ebo, Vito Sabato

The journal of allergy and clinical immunology. In practice 2023 Feb

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In light of the pandemic of spurious penicillin allergy, correct diagnosis of amoxicillin (AX) allergy is of great importance. The diagnosis of immediate hypersensitivity reactions relies on skin tests and specific IgE, and although reliable, these are not absolutely predictive. Therefore, drug challenges are needed in some cases, which contain the risk of severe reactions. Safe in vitro diagnostics as an alternative for the drug challenge in the diagnostic workup of AX allergy would be more than welcome to fill this gap. In this respect, the basophil activation test (BAT) has shown potential, but its clinical reliability is doubtful. To investigate the reliability of the BAT to AX and determining its exact place in the diagnostic algorithm of AX allergy. BAT for AX was performed in 70 exposed control individuals and 66 patients diagnosed according to the European Academy of Allergy and Clinical Immunology guidelines for AX allergy. Upregulation of both CD63 and CD203c was flow-cytometrically assessed. Analyses revealed that 1370 μmol/L and 685 μmol/L were the most discriminative stimulation concentrations for CD63 and CD203c upregulation, respectively, and a diagnostic threshold of 9% for positivity for both markers was identified. At these concentrations, sensitivity and specificity for CD63 upregulation were 13% and 100%, respectively, and for CD203c upregulation, 23% and 98%. BAT with dual analysis of CD63 and CD203c is of poor performance to document AX allergy. The sensitivity is too low to let it occupy a prominent role in the diagnostic algorithm. Copyright © 2022. Published by Elsevier Inc.

Citation

Kevin Heremans, Alessandro Toscano, Jessy Elst, Athina L Van Gasse, Christel Mertens, Michiel Beyens, Marie-Line M van der Poorten, Margo M Hagendorens, Didier G Ebo, Vito Sabato. Basophil Activation Test Shows Poor Sensitivity in Immediate Amoxicillin Allergy. The journal of allergy and clinical immunology. In practice. 2023 Feb;11(2):500-505