Structural and functional asymmetry of RING trimerization controls priming and extension events in TRIM5α autoubiquitylation.

Frank Herkules, Corey H Yu, Alexander B Taylor, Vi Dougherty, Susan T Weintraub, Dmitri N Ivanov

Nature communications 2022 Nov 19

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TRIM5α is an E3 ubiquitin ligase of the TRIM family that binds to the capsids of primate immunodeficiency viruses and blocks viral replication after cell entry. Here we investigate how synthesis of K63-linked polyubiquitin is upregulated by transient proximity of three RING domains in honeycomb-like assemblies formed by TRIM5α on the surface of the retroviral capsid. Proximity of three RINGs creates an asymmetric arrangement, in which two RINGs form a catalytic dimer that activates E2-ubiquitin conjugates and the disordered N-terminus of the third RING acts as the substrate for N-terminal autoubiquitylation. RING dimerization is required for activation of the E2s that contribute to the antiviral function of TRIM5α, UBE2W and heterodimeric UBE2N/V2, whereas the proximity of the third RING enhances the rate of each of the two distinct steps in the autoubiquitylation process: the initial N-terminal monoubiquitylation (priming) of TRIM5α by UBE2W and the subsequent extension of the K63-linked polyubiquitin chain by UBE2N/V2. The mechanism we describe explains how recognition of infection-associated epitope patterns by TRIM proteins initiates polyubiquitin-mediated downstream events in innate immunity. © 2022. The Author(s).

Citation

Frank Herkules, Corey H Yu, Alexander B Taylor, Vi Dougherty, Susan T Weintraub, Dmitri N Ivanov. Structural and functional asymmetry of RING trimerization controls priming and extension events in TRIM5α autoubiquitylation. Nature communications. 2022 Nov 19;13(1):7104