A novel biosensor-based method for the detection of p-nitrophenol in agricultural soil.

Zhao Ma, Yuanbo Li, Zhongyi Lu, Jie Pan, Meng Li

Chemosphere 2023 Feb

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Directly measurement of the bioavailable concentration of soil contaminants is essential for their accurate risk assessment. In this study, we successfully modified and identified the key genetic elements (pobR1-3) for the bio-detection of p-nitrophenol and synthesized five novel whole-cell biosensors (Escherichia coli BL21/pPNP-mrfp, E. coli BL21/pPNP-CFP, E. coli BL21/pPNP-YFP, E. coli BL21/pPNP-GFP, and E. coli BL21/pPNP-amilCP) to directly detect the concentration of p-nitrophenol in soils. These biosensor methods contained a simple biosensor activation and sample extraction step, a cost-effective detection means, and a fast detection process (5 h) by using a 96-microwell plate with a low background value and high-reliability equation for p-nitrophenol detection. These biosensors had a detection limit of 6.21-25.2 μg/kg and a linear range of 10-10000 μg/kg for p-nitrophenol in four soils. All biosensors showed better detection performance in the detection of p-nitrophenol in soil samples. The biosensors method can help to quickly and directly assess the actual bioavailable fractions of p-nitrophenol in soils, thus facilitating to understand the environmental cycling of p-nitrophenol. Copyright © 2022 Elsevier Ltd. All rights reserved.

Citation

Zhao Ma, Yuanbo Li, Zhongyi Lu, Jie Pan, Meng Li. A novel biosensor-based method for the detection of p-nitrophenol in agricultural soil. Chemosphere. 2023 Feb;313:137306